Package Services
| Package options | The service content | The delivered product | cycle |
Polyclonal package 1 （From protein antigens） | Customer provides antigen protein Protein will be checked by SDS-PAGE Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection | 1-2ml pre-bleed 40-60ml post-bleed Project datasheet including ELISA data | 10-12 weeks |
Polyclonal package 2 （From the protein antigen to antigen affinity purification） | Customer provides antigen protein Protein will be checked by SDS-PAGE Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation | 1-2ml pre-bleed 2-5mg affinity purified antibody Project datasheet including ELISA and dot blot data | 10-12 weeks |
Polyclonal package3 （From peptide design to antiserum） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection | 2-3mg antigen peptide 1-2ml pre-bleed 40-60ml post-bleed Project datasheet including ELISA data | 12-15 weeks |
Polyclonal package4 （From peptide design to antigen purified antibodies） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation | 2-3mg antigen peptide 1-2ml pre-bleed 2-5mg affinity purified antibody Project datasheet including ELISA and dot blot data | 12-15 weeks |
Polyclonal package5 （From plasmid to antiserum） | Customer provides expression vector Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection | 1mg antigen protein 1-2ml pre-bleed 40-60ml post-bleed Project datasheet including ELISA data | 12-15 weeks |
Polyclonal package6 （From plasmid to antigen purified antibody） | Customer provides expression vector Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation | 1mg antigen protein 1-2ml pre-bleed 2-5mg affinity purified antibody Project datasheet including ELISA and dot blot data | 12-15 weeks |
Polyclonal package7 （From cDNA to antiserum） | Customer provides protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Recombinant vector | 1mg protein 1-2ml pre-bleed 40-60ml post-bleed Project datasheet including ELISA data | 14-16 weeks |
Polyclonal package8 （From cDNA to antigen purified antibody） | Customer provides gene and protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification Dot blot confirmation Recombinant vector | 1mg protein 1-2ml pre-bleed 2-5mg affinity purified antibody Project datasheet including ELISA and dot blot data | 14-16 weeks |
Polyclonal package9 （Phospho-specific polyclonal antibody） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification | 2-3mg peptide/EA (non- & phosphor- peptide) 1-2ml pre-bleed 2-5mg antibody against unphospho-peptide 2-5mg phospho-specific antibody Project datasheet including ELISA and dot blot data | 13-16 weeks |
Polyclonal package10 （Lys mono-methylated specific polyclonal antibody） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification | 2-3mg peptide/EA (non- & mono-methylated peptide) 1-2ml pre-bleed 2-5mg antibody against unmethylated peptide 2-5mg Lys(Me) specific antibody Project datasheet including ELISA and dot blot data | 15-18 weeks |
Polyclonal package11 （Lys dimethylated specific polyclonal antibody） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize two New Zealand rabbits Titer the immunization by ELISA Post-bleed collection Antigen affinity purification | 2-3mg peptide/EA (non- & di-methylated peptide) 1-2ml pre-bleed 2-5mg antibody against unmethylated peptide 2-5mg Lys(Me2) specific antibody Project datasheet including ELISA and dot blot data | 15-18 weeks |
Monoclonal package1 （From protein to ascites purification） | Customer provides antigen protein Protein will be checked by SDS-PAGE Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Dot blot for QC. | 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones 2-5mg purified antibody from two hybridoma clones Project datasheet including ELISA, dot blot and isotype determination data | 18-24 weeks |
Monoclonal package2 （From peptide design to ascites purification） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Dot blot for QC. | 2-3mg peptides 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones 2-5mg purified antibody from two hybridoma clones Project datasheet including ELISA, dot blot and isotype determination data | 20-26 weeks |
Monoclonal package3 （From plasmid to ascites purification） | Customer provides expression vector lExpress and purify the antigen protein Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Dot blot for QC. | 1mg protein 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones 2-5mg purified antibody from two hybridoma clones Project datasheet including ELISA, dot blot and isotype determination data | 20-26 weeks |
Monoclonal package4 （From cDNA to ascites purification） | Customer provides gene and protein information We clone the gene and construct the expression vector for fusion protein Express and purify the antigen protein Immunize four mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Dot blot for QC. | recombinant vector 1mg protein 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones 2-5mg purified antibody from two hybridoma clones Project datasheet including ELISA, dot blot and isotype determination data | 22-26 weeks |
Monoclonal package5 （Phospho- specific monoclonal antibodies） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize six mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Dot blot for QC. | 2-3mg peptide including non- and phosphor-peptide 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones specific for phosphor-peptide 2 hybridoma clones for non-phospho-peptide 2-5mg purified phosphor-specific antibody from two hybridoma clones 2-5mg purified non-specific antibody from two hybridoma clones Project datasheet including ELISA, dot blot and isotype determination data | 21-26 weeks |
Monoclonal package6 （Western guaranteed monoclonal antibodies） | Customer provides antigen peptide information (or we provide candidate sequences for peptide antigen and discuss with customer. Customer will make his final decision) Synthesize one peptide (12-22aa, purity>85%) Conjugate the peptide to carrier protein (KLH, BSA or OVA) Immunize six mice (Balb/C) Titer the immunization by ELISA Hybridoma fusion and cloning Screening: at least one hybridoma clone which is positive to antigen (total five hybridoma clones). IgG isotype determination Ascites are prepared from two hybridoma clones which customer selects. Antigen affinity purification Western blot for QC. | 2-3mg peptides 50ul pre-bleed (1:10dilution) 2-5 hybridoma clones 2-5mg purified antibody from two hybridoma clones Project datasheet including ELISA, Western blot and isotype determination data | 20-26 weeks |
