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      1. Misc. Services

        Current Location>>Home>> >>Products >>Antibodies >>Misc. Services

        Protein antigen

        The service contentcycle
        Peptide coupling(KLH/BSA/OVA,≤5mg)2-3 days

        Protein antigen

        The service contentcycle
        The gene synthesis3-4 weeks

        PCR gene amplification

        (Provide the template,≤1000bp)
        2-3 weeks
        Carrier to build2-3 weeks
        Small amounts of expression1 week
        A large number of expression and purification2-3 weeks

        Polyclonal antibody production

        The service contentcycle
        The purity and concentration of the protein antigen detection(SDS-PAGE)2-3 days
        Immune serum collected before1 day
        Animal immunity and serum collection(Two New Zealand rabbit)10 week
        Animal immunity and serum collection(Two New Zealand rabbit,A rapid process)6-8 weeks
        Immune serum titer detection(ELISA)2-3 days
        Immune animal screening before(ELISA)2-3 days
        Immune animal screening before(Western Blot)1 week
        Immune animal screening before(Dot Blot)1 week
        Increase the immune rabbit(1 only)6-10 weeks
        Immune rabbit keep(1 only)1 week
        Increased serum collection(10ml,1 only)1 week
        Extend the immunity and serum collected(10ml,1 only)1 week
        Extend the immunity and serum collected(A goat)20 week
        Extend the immunity and serum collected(Two rats)10 week
        Extend the immunity and serum collected(Four mice)10 week
        Extend the immunity and serum collected(Two guinea pigs)11 week

        Monoclonal antibody production

        The service contentcycle
        The purity and concentration of the protein antigen detection(SDS-PAGE)2-3 days
        Immune serum collected before1 day
        Animal immunity and serum collection(Four mice)6-10 weeks
        Immune serum titer detection(ELISA)2-3 days
        Cell fusion and hybridoma preparation10-16 weeks
        Subtype identification1 week
        Ascites production(Three mice)3-4 weeks
        Transfer cells flask culture(1L)5-7 weeks

        Immune animal screening before

        (ELISA)
        2-3 days

        Immune animal screening before

        (Western Blot)
        1 week

        Immune animal screening before

        (Dot Blot)
        1 week
        Cloning maintain training(1 only)1 week
        Cloning of cryopreserved(1 only)1 week

        Antibody purification

        The service contentcycle
        Saturated ammonium sulfate purification antiserum(≤60ml)3-5 days
        Protein A/G affinity purification antiserum(≤60ml)3-5 days
        Antigen affinity column preparation2-3 days
        Antigen affinity purification antiserum(≤60ml)3-5 days

        Antibody detection

        The service contentcycle
        ELISA detection(1-10 samples)2-3 days
        Cell protein extraction(1 as a sample)2-3 days
        Tissue protein extraction(1 as a sample)2-3 days

        Determination of protein concentration

        (1 as a sample)
        2-3 days
        Antibodies to Western Blot detection(1 as a sample)1 week
        Antibodies are made in Western Blot detection(

        2-8 samples, provide testing sample concentration

        1 week
        Antibodies are made in Western Blot detection(2-8 samples, the customer does not provide samples)2-3 weeks
        On the basis of internal antibody detection, Western Blot detection target antibody(2-8 samples)1-2 weeks
        Dot Blot test(1-10 samples)2-3 days

        IHC testingProvide slices, 1 picture

        1 week

        Antibodies to tag

        The service contentcycle
        HRP labeling(≤5mg)1-2 weeks

        Biotin labeling(≤5mg)

        1-2 weeks


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